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bbsi digested pxr003 vector  (Addgene inc)


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    Structured Review

    Addgene inc bbsi digested pxr003 vector
    Bbsi Digested Pxr003 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/casrx+grna+cloning+backbone/pXR003%3A+CasRx+gRNA+cloning+backbone+(Plasmid+%23109053)/pm41928225-94-9-12
    Average 95 stars, based on 71 article reviews
    bbsi digested pxr003 vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: CD36 inhibition reduces non-small-cell lung cancer development through AKT-mTOR pathway
    Article Snippet: .. CasRx gRNA cloning backbone (pXR003, #109053, Addgene) was digested with BbsI and then ligated with annealed oligo duplex using T4 ligase. ..

    Article Title: AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected mice.
    Article Snippet: .. Immediately downstream of the expression cassette, we assembled in the gRNA backbone driven by human U6 promoter, a gift from Patrick Hsu (pXR003: CasRx gRNA cloning backbone, Addgene no. 109053), using Gibson Assembly reaction. ..

    Article Title: Deep screening of proximal and distal splicing-regulatory elements in a native sequence context
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Table 1.

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data .

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements.
    Article Snippet: The resulting dRfxCas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Cloning of individual gRNAs for transfection Oligonucleotides corresponding to individual gRNAswere synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data 1.

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: We amplified these plasmids using the DH5 α (Tolobio) competent cells. .. The dCasRx (Addgene plasmid no. 109050) and CasRx gRNA cloning backbone (Addgene plasmid no. 109053) were purchased from Addgene. ..

    Article Title: Lineage-specific splicing regulation of MAPT gene in the primate brain
    Article Snippet: .. The oligonucleotides were synthesized (IDT, Coralville, IA) and cloned into the CasRx gRNA cloning backbone using BsmBI restriction site (Addgene plasmid #138150). ..

    Article Title: Inducible RNA targeting and N 6 -methyladenosine editing by a split-Cas13 architecture
    Article Snippet: .. The CasRx (Addgene plasmid no. 109049), dCasRx (Addgene plasmid no. 109050), CasRx gRNA cloning backbone (Addgene plasmid no. 109053), PspCas13b (Addgene plasmid no. 103862), and PspCas13b gRNA cloning backbone (Addgene plasmid no. 103854) were purchased from Addgene. ..

    Expressing:

    Article Title: AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected mice.
    Article Snippet: .. Immediately downstream of the expression cassette, we assembled in the gRNA backbone driven by human U6 promoter, a gift from Patrick Hsu (pXR003: CasRx gRNA cloning backbone, Addgene no. 109053), using Gibson Assembly reaction. ..

    Synthesized:

    Article Title: Deep screening of proximal and distal splicing-regulatory elements in a native sequence context
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Table 1.

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data .

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements.
    Article Snippet: The resulting dRfxCas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Cloning of individual gRNAs for transfection Oligonucleotides corresponding to individual gRNAswere synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data 1.

    Article Title: Lineage-specific splicing regulation of MAPT gene in the primate brain
    Article Snippet: .. The oligonucleotides were synthesized (IDT, Coralville, IA) and cloned into the CasRx gRNA cloning backbone using BsmBI restriction site (Addgene plasmid #138150). ..

    Clone Assay:

    Article Title: Deep screening of proximal and distal splicing-regulatory elements in a native sequence context
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Table 1.

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements
    Article Snippet: The resulting d Rfx Cas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data .

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements.
    Article Snippet: The resulting dRfxCas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Cloning of individual gRNAs for transfection Oligonucleotides corresponding to individual gRNAswere synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data 1.

    Article Title: Lineage-specific splicing regulation of MAPT gene in the primate brain
    Article Snippet: .. The oligonucleotides were synthesized (IDT, Coralville, IA) and cloned into the CasRx gRNA cloning backbone using BsmBI restriction site (Addgene plasmid #138150). ..

    Transfection:

    Article Title: CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements.
    Article Snippet: The resulting dRfxCas13d-BFP (dCas13d-BFP) plasmid was confirmed using Sanger sequencing (Eton Bioscience Inc., Union, NJ). .. Cloning of individual gRNAs for transfection Oligonucleotides corresponding to individual gRNAswere synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites. .. All constructs were confirmed by Sanger sequencing (Eton Bioscience Inc., Union, NJ). gRNA targeting sequences can be found in Supplementary Data 1.

    Plasmid Preparation:

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: We amplified these plasmids using the DH5 α (Tolobio) competent cells. .. The dCasRx (Addgene plasmid no. 109050) and CasRx gRNA cloning backbone (Addgene plasmid no. 109053) were purchased from Addgene. ..

    Article Title: Lineage-specific splicing regulation of MAPT gene in the primate brain
    Article Snippet: .. The oligonucleotides were synthesized (IDT, Coralville, IA) and cloned into the CasRx gRNA cloning backbone using BsmBI restriction site (Addgene plasmid #138150). ..

    Article Title: Inducible RNA targeting and N 6 -methyladenosine editing by a split-Cas13 architecture
    Article Snippet: .. The CasRx (Addgene plasmid no. 109049), dCasRx (Addgene plasmid no. 109050), CasRx gRNA cloning backbone (Addgene plasmid no. 109053), PspCas13b (Addgene plasmid no. 103862), and PspCas13b gRNA cloning backbone (Addgene plasmid no. 103854) were purchased from Addgene. ..



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